Journal: Cell Reports Medicine

Article Title: A distinct innate immune signature marks progression from mild to severe COVID-19

doi: 10.1016/j.xcrm.2020.100166

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Article Snippet: EQ Four Element Calibration Beads , Fluidigm , Cat# 201078.

Techniques: Purification, Control, Recombinant, Blocking Assay, Electron Microscopy, Isolation, Enzyme-linked Immunosorbent Assay, Labeling, Mass Cytometry, Software, Diffusion-based Assay

Journal: eLife

Article Title: LIN37-DREAM prevents DNA end resection and homologous recombination at DNA double-strand breaks in quiescent cells

doi: 10.7554/eLife.68466

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Article Snippet: Lig4 −/− or Lig4 −/− :Lin37 −/− abl pre-B cells were transduced with lentiviral mouse genome-wide CRISPR gRNA library V2 (Addgene #67988) by centrifuging a cell and viral supernatant mixture (supplemented with 5 μg/ml polybrene) at 1800 rpm for 90 min. BFP-positive (stably transduced) cells were isolated on BD FACSAria II Cell Sorter, treated with 3 μg/ml doxycycline for 7 days followed by treatment with 3 μM imatinib for 2 days.

Techniques: Recombinant, Plasmid Preparation, Genome Wide, CRISPR, Clone Assay, Flow Cytometry, Staining, Sequencing, Software, Microscopy